EU regulations, the Austrian legislator and innovative research approaches require new dimensions for the implementation of the three Rs (3Rs). The 3Rs “Replacement”, “Reduction” and “Refinement” are guiding principles for more ethical use of animals in testing. A first initiative in this direction was the establishment of the Core Facility Alternative Biomodels and Preclinical Imaging belonging to the Division of Biomedical Research. The focus of this Core Facility is to set up alternative methods and give support by new alternative methods for all research developing at the Medical University of Graz.

 

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CellBank Graz

The CellBank Graz offers a wide range of services and support related to cell and tissue culture to all researchers at the Medical University of Graz. CellBank Graz is ISO 9001:2008 certified, thus ensuring state-of-the-art operating procedures with respect to the culturing and cry-preservation of cell lines and primary cells. The CellBank acts as a distributor for cell lines at the Medical University of Graz and as a secure repository for cell lines developed by local researchers. Our aim is to make it easier and cheaper for local researchers. New established and characterized cell lines (melanoma, glioblastoma, chordoma cell lines) are available for all researcher. As an additional service we offer Mycoplasma detection, STR analyses and virus detection.

Mycoplasma Testing

A common issue in cell culture is contamination. It is estimated that many established cell lines are contaminated by Mycoplasma. It is important for laboratories to check regulary their cell lines for Mycoplasma infection.

Identification Testing

A further common issue in cell culture is cross contamination with various cell lines. For the identification we use the Power-Plex® 16 system (Promega).

Virus Detection

Cell cultures may also be contaminated by other organisms like viruses. We offer the PCR detection of squirrel monkey retrovirus (SMRV) and Epstein-Barr virus (EBV).

  

Primary Cell Culture

Over the last three decades, protocols for primary cell cultures have been refined and have become an essential tool in biomedical research. Primary cells are taken directly from human or animal tissues, closely mimic the in vivo state and therefore generate more physiologically relevant data. We offer two isolation systems:

   

 

Manual dissociation

Tissue can be dissociated into single-cell suspension by mechanical dissociation and/or enzymatic digestion. A wide range of various enzymatic possibilities are available, while the choice of appropriate enzymes (collagenase, trypsin, pronase,dispase) needs to be adjusted for a particular type of cell isolation.

Gentle MACSTM semi-automated dissociation

The gentleMACSTM Dissociator is a benchtop instrument for the semi-automated dissociation of tissues into single-cell suspensions or thorough homogenates. The instrument offers optimized gentleMACS programs for a variety of specific applications. Special protocols have been developed for various tissues. The standardized tissue dissociation or homogenization procedures enable reliable and reproducible results.

 

3D Cell Culture

3D Cell Culture techniques more closely mimic natural tissues and organs than cells growth in 2D. 2D cells grow adherently and form unnatural cell attachments, whereas in 3D cell culture cells form natural cell-to-cell contacts. We offer hanging drops and spheroid cultivation for 3D systems.

 

The XCelligence System

The RTCA DP Instrument expands the throughput and application options of the xCELLigence Real-Time Cell Analyzer (RTCA) portfolio. Featuring a dual-plate (DP) format, the instrument measures impedance-based signals in both cellular and cell invasion/migration (CIM) assays – without the use of exogenous labels. With outstanding application flexibility, the RTCA DP Instrument supports multiple users performing short-term and long-term experiments

Molecular imaging is an emerging field in which the modern tools of molecular and cell biology are being married to state-of-art technology for non-invasive imaging. The goals of this field are to develop technologies and assays for imaging molecular and cellular events in living organisms. These approaches should help to lead to better methods for studying biological processes as well as diagnosing and managing diseases. At the moment three systems are available:

 

INVEON microCT

The MicroCT (Siemens) is designed to meet your throughput, resolution, and image quality needs, Inveon sets the standard for in-vivo preclinical micro computed tomography. With a range of X-ray source and detector configurations available, the ability to adjust the scanner magnification between scans, and a suite of image reconstruction and data analysis tools, the Inveon delivers the most versatile preclinical CT solution in its class.

Application: Bone structure analysis, Bone density, ..

 

 

VisualSonics’ Vevo 770

offers spatial resolution down to 30 microns. The system is noninvasive, which allows longitudinal study and reduces the number of mice required. VisualSonics technology provides scientific professionals with a simple method for efficiently viewing extremely small physiological structures and for imaging living tissue and blood flow with near-microscopic resolution. The Vevo 770 has been developed specifically for mice.

Application: Cardio-vascular research, Cancer research

 

 

 

 

 The CRi Maestro™ system

Our Maestro instrument is used for fluorescencebased in-vivo molecular imaging. Using multispectral acquisition and analysis, it provides dramatically improved sensitivity, multicolor flexibility and quantitative accuracy for both visible and near-infrared labels. We have two emission   filters available for imaging: 515 nm (blue) and 750 nm (red).

 

 

 

MaestroTM Filter Selection

 

     

 

Filter Set

 

Excitation

Filter

Emission

Filter

Acquisition

Settings

Fluorophore

 

Blue

 

445 to 490 nm

 

515 nm

longpass

500 to 720

in 10 nm steps

Alexa Fluor® 488,500,514,532,546,555
Cy2TM, Cy3
TM
GFP, FITC, eGFP, YFP, Rhodamine

Red

 

615 to 665 nm

 

700 nm

longpass

680 to 950

in 10 nm steps

Alexa Fluor® 750
Cy7TM

 

                                  

  Ass. Prof. PD Dr. Beate Rinner

  Head of the Core Facility

  beate.rinner@medunigraz.at

  Tel. +43 316 385 73524

 

 

 

 

Cell Bank and Alternative Biomodels

 

  Katharina Meditz, BSc.

  Technician

  katharina.meditz@medunigraz.at

  Tel. +43 316 385 73501

 

 

  Marie-Therese Frisch, MSc.

  Technician

  m.frisch@medunigraz.at

  Tel. +43 316 385 73516

 

 

 

Preclinical Imaging

 

 Ines Anders, MSc.

 ina.anders@medunigraz.at

 Tel. +43 316 385 72732

 

 

 

 

 Alexandra Lipfert, MSc.

 radiology technician

 a.lipfert@medunigraz.at

 Tel. +43 316 385 72732

 

 

PhD Students

 

 Alexander Stallinger, MSc.

 alexander.stallinger@medunigraz.at

 Tel. +43 316 385 72732

 

  

Price

  MUG intern 

Price

  MUG extern   

Cell lines    
Common Cell lines € 50 not available
MUG Chor 1 € 150 € 350
MUG Mel 1 € 150 € 350
MUG Mel 2 € 150 € 350
MUG CC 1 € 150  € 350 
     
Mycoplasma testing    
Mycoplasma PCR € 17,80 € 45
     
Virus testing    
EBV testing € 15 € 45
SMRV testing € 15 € 45
     
Identification testing    
STR analysis € 58,40 € 150
     
  Price per hour  Price per hour 
 Optical Imaging €   0,13 € 13,77
 Micro CT € 36,20 € 77,67
 Micro Ultrasound €   0,40 € 16,77
     

 

Cell based assays

  • Apoptose induction with Staurosporin
  • Electroporation
  • Reverse transfection of cells with siRNA
  • Synchronisation of cells
  • Colony forming units (CFU's)
  • Tumorigenicity 

Cell counting

  • Electronic counting
  • Cell counting with hemocytometer

 Culturing of cells

  • Culturing
  • Thawing
  • Cryopreservation

 Identification testing: STR PCR

 Virus detection: EBV, SMRV

 Mycoplasma detection

  • Mycoplasma screening using PCR
  • MycoAlert

 Primary cell culture

  • Precoating
  • tumor cells (melanoma, glioblastoma, chordoma)
  • non-tumorigenic:
  • fibroblasts, melanocytes, keratinocytes, preadipocytes

 Proliferation and viability: ATP, LDH, MTS, BrDu, Thymidine

 XCelligence

  • Cell invasion and migration assays
  • compound- and cell-mediated cytotoxicity
  • cell adhesion and cell spreading
  • cell proliferation and cell differentiation
  • receptor mediated signaling
  • virus-mediated cytopathogenicity
  • continous quality control of cells

microCT

  • Bonestructure Analysis: Osteoporosis/Bonedensity
  • Ex-vivo studies: Biomaterial
  • Dental research
  • Lung inflammation research

micro Ultrasound

Vascular research

  • real time blood flow evaluation
  • Visualization of vessels & quantification of abnormalities
  • Identify & quantify the presents of plaques

Cardiology research

  • Visualization & quantification of cardiac function
  • Embryonic lethal screening

Cancer research

  • Evaluation & volumetric sizing of subcutaneous tumors
  • monitoring of therapeutic effectiveness longitudinally

Developmental biology

  • Real time evaluation & quantification of functional
  • and anatomical target in the developing small animals

optical Imaging

  • Tumor monitoring