CytoFLEX LX

The CytoFLEX LX (Beckman Coulter) is a compact 5-laser benchtop analytical flow cytometer for multicolor analysis.

Up to 19 fluorescence parameters and 2 scatter parameters (FSC and SSC) can be measured simultaneously from five different excitation wavelengths (355 nm, 405 nm, 488 nm, 561 nm and 638 nm). An additional side scatter (VSSC) from the violet (405 nm) laser allows the detection of very small particles (<200 nm). The emitted light is detected through an innovative Wavelength Division Multiplexing (WDM) detection module, which includes high efficiency and low-noise Fiber Array Photodiode Detectors (FAPDs) for superior resolution and sensitivity. At a speed of up to 30.000 events per second with 21 selected parameters even large quantities of cells can be measured quickly. Sample volumes as low as 10µl can be used.

The fully digital system provides a 7 decades tunable dynamic range and is operated by a PC with Windows 10 operating system using easy-to-operate, intuitive CytExpert acquisition/analysis software which allows compensation to be performed both manually and automatically. Due to the linear response across the entire range compensation values are automatically adjusted in real-time if the gain settings are changed using a compensation library (a repository of compensation spillover values of dyes). The advanced optical system is alignment free. The laser delays are automatically adjusted by the daily QC.

 
 
 
 
 
 
 
 
 
 
 
 
 
 

CytoFLEX S

The CytoFLEX S (Beckman Coulter) is a compact 4-laser benchtop analytical flow cytometer equipped with a plate loader for multicolor analysis.
Up to 13 fluorescence parameters and 2 scatter parameters (FSC and SSC) can be measured simultaneously from four different excitation wavelengths (405 nm, 488 nm, 561 nm and 638 nm). An additional side scatter (VSSC) from the violet (405 nm) laser allows the detection of very small particles (<200 nm).

The emitted light is detected through an innovative Wavelength Division Multiplexing (WDM) detection module, which includes high efficiency and low-noise Fiber Array Photodiode Detectors (FAPDs) for superior resolution and sensitivity. At a speed of up to 30.000 events per second with 15 selected parameters even large quantities of cells can be measured quickly. Sample volumes as low as 10 µl can be used.
The fully digital system provides a 7 decades tunable dynamic range and is operated by a PC with Windows 7 operating system using easy-to-operate, intuitive CytExpert acquisition/analysis software which allows compensation to be performed both manually and automatically. Due to the linear response across the entire range compensation values are automatically adjusted in real-time if the gain settings are changed using a compensation library (a repository of compensation spillover values of dyes).
The advanced optical system is alignment free. The laser delays are automatically adjusted by the daily QC.

 

 

FACS Aria IIIu

The FACSAria IIIu (BD Biosciences) is a high–speed digital benchtop flow cytometer for cell sorting. It is engineered with a fixed–alignment closed–cuvette flow cell providing superior fluorescence sensitivity over traditional jet–in–air cell sorters. 
Our Aria is configured with four lasers: a red  laser exciting at 633 nm, a blue laser exciting at 488 nm, a yellow-green laser exciting at 561nm, and a violet laser exciting at 405 nm. It is equipped with 13 fluourescence detectors, thus allowing 15 parameters (two scatter parameters and 13 fluorochromes) to be measured concurrently. It can sort in four directions at rates up to 25.000 cells per second at > 98% purity with a maximum acquisition rate of 70.000 events per second (with 8 parameters).

BD FACS Accudrop is integrated into the system allowing for quick and accurate drop–delay determination. Sort samples can be collected in two–way or four–way bulk sorting devices with a variety of tube sizes or via Automatic cell deposition unit (ACDU) for microplate and slide sorting. The FACSAria is also equipped with the BD Aerosol Management System for sorting of potentially biohazardous samples. The FACSAria is operated by a PC with Windows 7 operating system using FACSDiva8 acquisition/analysis software.



 

 

 

 

 

 

 

 

 

 

 

 

NanoSight NS300

The NanoSight NS300 (Malvern) is a laser-based instrument which uses the properties of light scattering and Brownian motion in order to obtain the size distribution and concentration measurement of nanoparticles (fluorescent and non-fluorescent) from 10 nm to 2000 nm in liquid suspension. Two lasers are available: a violet laser exciting at 405 nm (longpass filter: 430 nm) and a blue laser exciting at 488 nm (longpass filters: 500 nm or 565 nm). The laser beam is passed through the sample chamber and the particles in suspension scatter light so that they can be visualized via microscope onto which is mounted a camera. The camera operates at 30 frames per second, capturing a video file of the particles moving under Brownian motion. The software tracks particles individually using the Stokes-Einstein equation to calculate their hydrodynamic diameters. A syringe pump ensures continuous sample flow and improves the statistical significance.

 

 

Bio-Plex 200

The Bio–Plex 200 multiplex suspension array system is a complete biomarker assay system employing Luminex xMAP–technology for the simultaneous detection and quantitation of multiple bioanalytes (proteins, peptides, DNA, RNA) in a single microplate well requiring only very small sample volumes.
It is a fully integrated and validated system combining hardware, Bio–Plex Manager software, and system validation and calibration tools. The system uses a liquid suspension array of microscopic beads that are conjugated with different capture molecules. The beads are color–coded with different ratios of two spectrally distinct fluorophores.

The array reader is quipped with two lasers: a red classification laser (635 nm) for bead discrimination and a green reporter laser (532 nm) for detection and quantitation of captured analytes. Potential applications include immunoassays (cytokines, phosphoproteins), enzyme assays, receptor–ligand assays and DNA⁄RNA hybridization assays.

 

Heimo Strohmaier, Ph.D.

Head

Phone: +43 316 385 73513

E-Mail: heimo.strohmaier@medunigraz.at

 

 

 

 

 

Jennifer Ober, B.Sc.

Application Specialist

Phone: +43 316 385 73521

E-Mail: jennifer.ober@medunigraz.at

 

 

 

 

 

 

Verena Zrim, B.Sc.

Application Specialist

Phone: +43 316 385 80993

E-Mail: verena.zrim@medunigraz.at

 

We have experience with the following assays:

Analytical Flow Cytometry:

· DNA-Analysis:

    - Cell cycle (PI, Phospho-Histone H3, Pyronine Y)

    - Ploidy/DNA-index

    - Cell proliferation (BrdU, Click-iT)

· Apoptosis:

    - Annexin V

    - Cleaved Caspase-3

    - Sub-G1

    - Mitochondrial membrane potential (JC-1)

    - DNA-fragmentation (TUNEL)

· Immunology:

    - Immunophenotyping (B, T, NK/T, monocytes, granulocytes)

    - Regulatory T-cells (Tregs)

    - Dendritic cells (DCs)

    - Progenitor/stem cells

· Others:

    - Viability/cytotoxicity

    - Characterisation of primary cells and cell lines

    - Tumor side population (Hoechst, Aldh+)

    - Lipid staining (Bodipy, Filipin)

Cell Sorting:

· Multiple bulk and single cell sorting (clonal expansion)

· Immune cells

· Progenitor/stem cells

· Transfected/transduced cells (GFP, YFP, etc.)

· Cell size sorting

Multiplexing (Luminex):

· Extracellular cytokines (human and mouse)

 

 

 

 

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