Created by: Gerhard   Created on: 2017-06-07T08:33:37.390+0200   Modified by: Sara   Modified on: 2019-03-06T11:08:07.807+0100
Genomics and Transcriptomics at the University of Veterinary Medicine Vienna   The Core Facility  Genomics & Transcriptomics  at the  University of Veterinary Medicine  Vienna  focuses on the design and accurate measurement of quantitative experiments targeting DNA and RNA (mRNA to miRNA) sequences.Sample (pre)processing, for example by separating single cells using fluorescence activated cell sorting and by laser capture micro-dissection (LCM) to facilitate selective sampling of individual cells or groups of cells from histological specimens, represents a special field of expertise. The amplification refractory mutation system (ARMS)-qPCR is a key method to quantify mixtures of SNP sequences. The facility offers the opportunity to run digital PCR to accomplish more accurate quantification compared to qPCR. Quantification of nucleic acid sequences can be performed by RT-qPCR (in house) or by several next-generation sequencing approaches (out...
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Created by: Gerhard   Created on: 2015-09-09T14:18:46.096+0200   Modified by: Sara   Modified on: 2019-03-06T11:03:33.479+0100
The Core Facility Genomics of the Medical University of Vienna operates as a full-service genomics facility providing research scientists and clinicians with services for high throughput analyses of genomes and transcriptomes, in close collaboration with the Biomedical Sequencing Facility of the CeMM / the Austrian Academy of Sciences. The Core Facility Genomics performes routinely RNA expression profiling and DNA variation analysis of samples of human, mouse and other organisms. Among others, we operate Illumina technologies for sequencing and Affymetrix GeneChip technologies. Our services include project design, wet-lab experimental procedures as well as data analysis and help with the interpretation of results.   Contact: https://corefacilities.meduniwien.ac.at/en/genomics/    
Created by: Gerhard   Created on: 2017-06-07T08:35:11.338+0200   Modified by: Gerhard   Modified on: 2017-06-07T08:35:11.338+0200
Genomics & Transcriptomics at the BOKU   The Extremophile Center Core Facility is specialized on a group of fungi, which are small, black and strong:  "Black yeasts" and "Microcolonial fungi" resist extreme situations; the UV-radiation and dehydration in desert just like the radioactivity or extreme ozone pollution. We examine the molecular and cellular mechanisms of stress resistance in fungi and other microorganisms with the aim to make them useful for biotechnological applications.   Our research aims to have biotechnological applications: Development of antioxidants and other cell-protectants for medical and cosmetic applications in relation to UV-stress and free radicals as well as in terms of application in radiotherapy. Development of novel anti-fungal therapies. Biotechnological use of novel degradation processes for biological purification of waste gases (biofilter), water and soil as well as the bioconversion of pollutants and other was...
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Created by: Gerhard   Created on: 2015-07-03T13:19:02.931+0200   Modified by: Gerhard   Modified on: 2016-01-28T15:30:01.415+0100
Core Facility Molecular Biology Graz [[Genomics_and_Transcriptomics_at_the_University_of_Veterinary_Medicine_Vienna]] [[Genomics and Transcriptomics at the AIT]] [[Genomics and Transcriptomics at the MUI]] [[Core Facility Genomics at Medical University of Vienna]]
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Created by: Gerhard   Created on: 2015-07-03T13:23:38.733+0200   Modified by: Stefan   Modified on: 2015-10-27T09:47:45.652+0100
Genomics and Transcriptomics at the MUI Sequencing & Genotyping Core Facility The Sequencing & Genotyping Core Facility of the Innsbruck Medical University focuses on high throughput DNA sample processing, SNP genotyping, real-time PCR, Sanger sequencing and analysis of mitochondrial DNA using both Sanger and next-generation sequencing. Sanger sequencing and fragments analysis applications are performed on an ABI 3130xl (16 capillaries; medium throughput) and a ABI 3730s (48 capillaries; compatible with both 96 and 384 well plates for high throughput). TECAN robots with either 8 pipetting channels or 96 channels are available for automated DNA sample normalization and high-throughput processing of 96 well or 384 well plates. SNP genotyping can be performed either on an ABI QuantStudio 6 Real-Time PCR system for low-throughput singleplex applications or an Agena (formerly Sequenom) MassARRAY4 DNA MALDI-TOF system  for large scale multiplexed geno...
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Created by: Gerhard   Created on: 2015-07-03T13:22:56.326+0200   Modified by: Marie   Modified on: 2015-10-21T14:19:58.325+0200
  Genomics and Transcriptomics at the AIT      The Business Unit Molecular Diagnostics at the AIT Austrian Institute of Technology focuses on the discovery and validation of biomarkers suitable for minimally invasive disease diagnosis – and/or prognosis from body fluids. Beside serum - and plasma-based diagnostic assay development the research group recently has a special interest in saliva diagnostics thereby focusing in particular on DNA-methylation-, micro-RNA - and autoantibody-based biomarkers. AIT’s technological expertise comprises the isolation of the whole variety of biomolecules from minimal amounts of sample material (e.g. biopsies, FFPE specimens , body fluids) as well as the profiling, discovery and validation of DNA-, mRNA/microRNA-, protein– or autoantibody-based biomarkers using various state-of-the-art high throughput technologies.   Devices: 1)    Ion Torrent PGM &...
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Created by: Bettina   Created on: 2014-07-02T12:10:23.237+0200   Modified by: Gerhard   Modified on: 2015-07-03T13:56:41.655+0200
Genomics and Transcriptomics at the Medical University Graz     The Core Facility Molecular Biology at the Medical University Graz focuses on Microbiome analysis by 16s amplicon based sequencing as well as on de novo and re-sequencing of small genomes on the MiSeq Illumina Next Generation Sequencing platform. Implementation of 16s analysis for clinical applications is on high priority of the group but the analysis from a variety of human, animal and environmental sample material is possible. The workflow offers automated DNA isolation, amplification of hypervariable regions, library preparation, sequencing and basic data analysis. Further, the Core Facility is very well experienced in gene expression analysis by the Affymetrix GeneChip ® array hybridization system, qRT-PCR and the nCounter ® Analysis System. The Affymetrix platform offers a highly reproducible whole transcriptome screening method and generated data are analyze...
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Created by: Gerhard   Created on: 2015-07-03T13:22:11.777+0200   Modified by: Gerhard   Modified on: 2015-07-03T13:22:11.777+0200
Genomics and Transcriptomics at the University of Veterinary Medicine Vienna   The Core Facility  Genomics & Transcriptomics  at the  University of Veterinary Medicine  Vienna  focuses on the design and accurate measurement of quantitative experiments targeting DNA and RNA (mRNA to miRNA) sequences. Sample (pre)processing, for example by separating single cells using fluorescence activated cell sorting and by laser capture micro-dissection (LCM) to facilitate selective sampling of individual cells or groups of cells from histological specimens, represents a special field of expertise. The amplification refractory mutation system (ARMS)-qPCR is a key method to quantify mixtures of SNP sequences. The facility offers the opportunity to run digital PCR to accomplish more accurate quantification compared to qPCR. Quantification of nucleic acid sequences can be performed by RT-qPCR (in house) or by several next-generation sequencing approac...
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