Metabolomics is the science of metabolites from endogenous and exogenous sources. The main categories of metabolites encompass lipids, amino acids, carbohydrates, nucleotides, catecholamines and exogenous pharmaceuticals. Due to the sheer vastness of the number of lipids, lipidomics is a separate scientific field as explained below.

Lipidomics is a constantly growing research area which extends into the realms of energy metabolism, membrane biology and signaling processes. The lipidome comprises a plethora of estimated 100,000 – 200,000 molecular compounds, many of them as of now presumably undetected. The scope of molecular structures reveals a surprisingly vast diversity ranging from commonly known triglycerides and phospholipids all the way to signaling compounds like steroids, S1P or eicosanoids and even rare species like microbial mycolic acids or highly complex glycolipids.

                                                                         

 

Read more about the methods , projects and services offered by the Core Facility Mass Spectrometry at the Medical University of Graz (MUG)

 

The protein facility established at Innsbruck Medical University is dedicated to provide investigators with equipment, expertise and custom services for the detection, characterization and quantification of proteins and peptides on a recharge basis. The facility maintains a suite of state of the art instrumentation including a MALDI TOF/TOF 4800 plus Analyzer (Applied Biosystems), a Q Exactive HF (ThermoScientific), a Hybrid FT-Mass Spectrometer LTQ Orbitrap XL ThermoScientific), an LTQ VELOS (ThermoScientific), a Procise 492 Protein Sequencer (Applied Biosystems), Nano-LC Gradient Systems UltiMate 3000 (Dionex), a Probot Microfraction Collector (LC-Packings) for on-line MALDI target preparations. Various Capillary Electrophoresis and HPLC Systems and, in addition, a Solaar M6 Dual Zeeman Spectrometer (ThermoScientific) for trace element analysis are operated in the facility.

Contact: Herbert Lindner (herbert.lindner@i-med.ac.at)

 

 

Metabolomics is an integral part of the broader field of systems biology and has attracted increasing interest for studies in important life science disciplines such as pharmacy, toxicology and medicine.

The metabolome is the set of all endo- and exogenous small molecular mass organic compounds (Mr < 1500) found in a given biological media. Asmetabolites are the ultimate downstream products of genomic, transcriptomic, and/or proteomic perturbations, changes in metabolite concentration and/or flux can reveal biologically relevant changes to the system. The primary task of metabolomics is the qualitative and quantitative analysis of the plurality of metabolites to identify markers or signatures that are characteristic for a defined biological system (“phenotype”).

Mass spectrometry usually hyphenated with chromatographic methods is a key analytical technology in metabolomics research.

 

Design of metabolomics studies

A metabolomics study frequently involves the comparative analysis of sample sets from a normal state and a perturbed state, where the perturbation can be of any nature, such as genetic variation, administration of a drug, or change in diet or lifestyle.

Metabolic profiling (targeted metabolomics) is the multiparallel relative quantification of defined sets of structurally known and biochemically annotated metabolites.

Metabolic fingerprinting is an unbiased approach aimed to characterize as many components of a biological system as possible.

 

Related areas of research

As the metabolome includes all small molecules introduced and modified by diet, medication, environmental exposure, and coexisting organisms,therapeutic drug monitoring, pharmacokinetics, and toxicology are regarded as research areas closely related to metabolomics.

 

Core Facility Metabolomics of the Medical University of Innsbruck

The mission of the Core Facility Metabolomics is to serve as an enabling resource for research and development programs. We aim to provide expertise and state-of-the-art technologies for the qualitative and quantitative analysis of small bioorganic molecules. Common targets are drugs, pharmaceuticals, endogenous compounds, and metabolites thereof included in all kinds of biological samples (e.g. biofluids, cells, tissues). The analytical method of choice is mass spectrometry (MS). Usually MS is hyphenated to chromatographic methods (liquid chromatography or gas chromatography).

 

A detailed description of projects, methods and services is available on our webpage.  

http://gerichtsmedizin.at/bioanalytical_mass_spectrometry.html

 

For more detailed information please contact

Assoc.Prof. Dr. Herbert Oberacher

Institute of Legal Medicine and Core Facility Metabolomics

Medical University of Innsbruck

Muellerstrasse 44

6020 Innsbruck

Tel: +43-512-9003-70639

Email: herbert.oberacher@i-med.ac.at

The Proteomics Unit as part of the VetCORE Facility for Research at the University of Veterinary Medicine Vienna provides researchers with equipment and know-how for protein identification and relative protein quantitation by mass spectrometry. Shotgun identification of complex samples and quantitation are performed with a Q-TOF high resolution mass spectrometer (TripleTOF 5600, Sciex) coupled to a nanoLC system (Ultimate 3000 RSLC, Dionex). For identification of 2D gel spots a MALDI-TOF-TOF instrument (Ultraflex II, Bruker) is used. Additionally, a spotter (Probot, Dionex) is available for special applications like DeNovo-Sequencing with LC-MALDI.

For further information visit our webpage:

www.vetcore.at

     

Contact information:

Ao. Univ.-Prof. Dipl.-Ing. Dr. Ebrahim Razzazi-Fazeli
+43 1 25077-3216
+43 664 60257-6381
e-mail: Ebrahim.Razzazi@vetmeduni.ac.at

 

 

Core Facility Proteomics at Medical University of Vienna

 

Protein Analysis Services and quantitative Method Development

The mission of the Proteomics Core Facility at the Medical University of Vienna is to provide the researchers at the Medical University of Vienna with consulting, teaching, protein analysis services and quantitative method development. Further, novel chromatographic separation methods are developed for both peptide and protein separations. These methods employ separation techniques, which are usually not used for biomedical or biochemical analysis but offer a great potential for improving detection rates for proteins in complex samples.

The Proteomics Core Facility provides a full proteomics infrastructure for the identification and characterization of proteins. The key technology of the Proteomics Core Facility is mass spectrometry for the qualitative and quantitative analysis and characterization of proteins. We primarily use the “bottom-up” proteomics approach, where all proteins are proteolytically (in most cases with tryptic, but other enzymes are also used) digested, producing peptide surrogates (signature peptides) of the original proteins.

Protein identifications are made using state-of-the-art search engines such as Mascot or X!Tandem, with the capability of searching standard or custom protein databases.

contact

Ass.-Prof. Mag. Dr. Goran Mitulovic

email: goran.mitulovic@meduniwien.ac.at

phone: + 43 (0)1 40400 - 73750, 53590

http://corefacilities.meduniwien.ac.at/proteomics/

 

Mass Spectrometry Core Facility at the BOKU

At the Cellular Analysis Core Facility advanced facilities for cell sorting and analysis enable studies in all “-omics” fields at one single location. High-speed cell sorting allows the isolation of desired cell types. Nano-LC-MS/MS is used for studies requiring proteomics and glycoproteomics data, while LC-MS/MS, GC-MS/MS, and high-resolution mass spectrometry platforms serve studies within the field of metabolomics.

Studies at Cellular Analysis focus on metabolomics and fluxomics for a wide range of applications. Within metabolomics, the major aims are targeted metabolic profiling of selected metabolites and the non-targeted analysis of all metabolites present in a biological system or compartment. Within fluxomics, which addresses the quantitative assessment of molecular fluxes in a biological system, the goal is the accurate determination of isotopologue or isotopomer ratios for the metabolites of interest.

Equipment:

  • Nano-LC-MS/MS
  • The Thermo Vantage Triple Quadrupole LC-MS/MS
  • The Thermo TSQ Quantum XLS Ultra Triple Quadrupole
  • GC-MS/MS
  • The Agilent 7200 GC-qTOFMS
  • The Agilent 6560 LC-IMS-qTOFMS
  • The Agilent 6230 LC-TOFMS
  • High-speed Cell Sorter

You find more detailed information about our facilities and services here: http://eq-vibt.boku.ac.at

For more information please contact:

Ingrid Döller-Diem

Tel.: +43 (0)1 47654-99304

Email: ingrid.doeller-diem@boku.ac.at

[[Lipidomics and Metabolomics in Graz]]

[[Mass Spectrometry in Innsbruck]]

[[Proteomics at the University of Veterinary Medicine in Vienna]]

[[Proteomics at Medical University of Vienna]]

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