Proteomics at Medical University of Vienna
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Core Facility Proteomics at Medical University of Vienna

 

Protein Analysis Services and quantitative Method Development

The mission of the Proteomics Core Facility at the Medical University of Vienna is to provide the researchers at the Medical University of Vienna with consulting, teaching, protein analysis services and quantitative method development. Further, novel chromatographic separation methods are developed for both peptide and protein separations. These methods employ separation techniques, which are usually not used for biomedical or biochemical analysis but offer a great potential for improving detection rates for proteins in complex samples.

The Proteomics Core Facility provides a full proteomics infrastructure for the identification and characterization of proteins. The key technology of the Proteomics Core Facility is mass spectrometry for the qualitative and quantitative analysis and characterization of proteins. We primarily use the “bottom-up” proteomics approach, where all proteins are proteolytically (in most cases with tryptic, but other enzymes are also used) digested, producing peptide surrogates (signature peptides) of the original proteins.

Protein identifications are made using state-of-the-art search engines such as Mascot or X!Tandem, with the capability of searching standard or custom protein databases.

contact

Ass.-Prof. Mag. Dr. Goran Mitulovic

email: goran.mitulovic@meduniwien.ac.at

phone: + 43 (0)1 40400 - 73750, 53590

http://corefacilities.meduniwien.ac.at/proteomics/